ISO INTERNATIONAL STANDARD 19040-1 First edition 2018-08 Water quality Determination of the estrogenic potential of water and waste water Part 1: Yeast estrogen screen (Saccharomyces cerevisiae) Qualitéde I'eau-Détermination du potentiel oestrogeniquede I'eau etdeseauxrésiduaires Partie 1:Essai d'oestrogénicité sur levures (Saccharomyces cerevisiae) Reference number IS0 19040-1:2018(E) [So @ IS0 2018 Copyright Intemational Organization for Standardization IS019040-1:2018(E) COPYRIGHTPROTECTEDDOCUMENT IS02018 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch.de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 0111 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland Copyrightilhlmafinal Organization fce Standardzation IS0 2018 -All rights reserved IS0 19040-1:2018(E) Contents Page Foreword ..V 1 Scope ..1 2 Normativereferences 3 Terms and definitions 4 Principle 5 Interferences 6 Apparatus and materials. ..5 7 Reagents, media and test strain .6 8 Sampling and samples .10 8.1 General .10 8.2 Bottles and material for sampling .10 8.3 Bottles and material pre-cleaning .10 8.4 Sampling procedure .10 8.5 Transport of samples. .11 8.6 Pretreatment of samples ..11 8.7 Storage of samples. .11 9 Procedure. .12 9.1 Preparation ofcryo-cultures forlong-term storage ..12 9.2 Overnight culture .12 9.3 Test set upfor aqueous samples .12 9.3.1 Preparation. .12 9.3.2 Preparation of the reference dilution series .12 9.3.3 Negative control .13 9.3.4 Blank replicate. .14 9.3.5 .14 Sampledilution 9.3.6 Field blank. .14 9.3.7 Plate setup. ..14 9.3.8 Inoculation of the test plate .14 9.4 Measurement. ..15 9.4.1 Measurement ofthe cell density .15 9.4.2 Measurement ofthereporter gene activity ..16 9.5 Calculationofthecorrectedabsorbanceandthereportergeneinduction .16 9.6 Calculation of therelativegrowth ..17 9.7 Estimation of the ECso of the reference compound by linear interpolation. ..17 10 Validity criteria .17 11 Assessmentcriteria ..18 12 Test report ..18 AnnexA (normative)Strain selection .19 Annex B (informative) Plate set up ..20 AnnexC (informative)Schemeoftestprinciple .21 AnnexD(informative)Testsetupforchemicalsandextracts .22 AnnexE(informative)Preparationofdilutionseries .26 Annex F (informative) Performance data ..27 AnnexG (informative)Useofotheryeaststrainsbased on Saccharomycescerevisiae ..40. AnnexH (informative)Statisticalassessment ..43 ii Copyrigh Intemational orgSi21&rAlh rights reserved